Journal: Integrative Cancer Therapies

Article Title: Anti-Cancer Effects of the Ethyl Acetate Fraction From Opuntia humifusa on Human Triple-Negative Breast Cancer Cells

doi: 10.1177/15347354251401187

Figure Lengend Snippet: Effects of the ethyl acetate fraction of O. humifusa on metastasis- and hypoxia-associated proteins in MDA-MB-231 cells. (A) MDA-MB-231 cells were treated with control, DMSO, or the ethyl acetate fraction of O. humifusa (150 and 250 μg/mL) for 48 hours. Protein expression levels of MMP-7, MMP-9, HIF-1α, and integrin β1 were assessed. (B) Protein relative expression of MMP-7, MMP-9, HIF-1α, and integrin β1. Data are presented as mean ± SD (n = 3). *** P < .001 versus DMSO-treated group.

Article Snippet: Primary antibodies used were as follows (all 1:1000 dilution): GAPDH (Cell Signaling Technology Cat# 2118, RRID: AB_561053), cleaved caspase-3 (Cat# 9664, RRID: AB_2070042), cleaved caspase-8 (Cat# 9496, RRID:AB_561381), cleaved caspase-9 (Cat# 7237, RRID: AB_10895832), pro-caspase-3 (Cat# 9662, RRID: AB_331439), pro-caspase-8 (Cat# 4790, RRID: AB_10545768), pro-caspase-9 (Cat# 9502, RRID:AB_2068621), ERK1/2 (Cat# 9102, RRID: AB_330744), Akt (Cat# 9272, RRID: AB_329827), lamin A/C (Cat# 2032, RRID: AB_2136278), HIF-1α (Cat# 3716, RRID: AB_2116962), integrin β1 (Cat# 4706, RRID: AB_823544), MMP-7 (Cat# 71031, RRID: AB_2799796), MMP-9 (Cat# 3852, RRID: AB_2144868), Smac/DIABLO (Cat# 15108, RRID: AB_2798711), cyclin A2 (Cat# 67955, RRID: AB_2909603), CDK4 (Cat# 12790, RRID: AB_2631166), and CDK2 (Cat# 2546, RRID: AB_2276129), all from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Control, Expressing